(1)
EMBL, 69 012 Heidelberg, Germany;
rost@columbia.edu;http://www.columbia.edu/~rost/
contact e-mail:rost@columbia.edu
When are two naturally evolved proteins likely to have similar structures?
Originally, Sander & Schneider defined a length-dependent cut-off for significant pairwise sequence identity. I refined their analysis on a much larger data set, and re-defined thresholds for pairwise sequence idenity and pairwise sequence similarity (unpublished).
Here I give a table for the old and the new thresholds.
The HSSP-curve was originally defined by (Sander & Schneider, 1991):
(1)
where L gave the number of residues aligned between two
proteins; pI the
cut-off percentage of identical residues over the L aligned
residues; and n described the distance in percentage points
from the curve (n = 0 corresponds to the original HSSP-curve;
n = 5 to the official HSSP database releases (Schneider et al., 1997)).
Although the absolute value for the threshold of the HSSP-curve was questioned, the functional shape proved basically correct. However, the larger data set analysed here revealed several problems in detail. First, a threshold of 25% was not reasonable for alignment length lower than about 150-200. Second, above alignment length of 100, the derivative of the curve separating true and false positives should be lower than at lengths below 80. I attempted to solve these problems by defining the following curve for the separation true and false positives:
(2)
where L gave the number of residues aligned between two
proteins; pI the
cut-off percentage of identical residues over the L aligned
residues; and n described the distance in percentage points
from the curve. The particular functional form guaranteed an
approximate saturation for long alignments. For alignments shorter
than 13 residues eq. 2 yielded values above 100%. This was acceptable
as 100% identical residues for poly-peptides of 10-11 residues
does NOT imply structural similarity between these peptides (Cohen et al.,
1993; Cerpa et al., 1996; Minor & Kim, 1996; Muñoz &
Serrano, 1996).
Compiling sequence identity neglects the physico-chemical nature of amino acids. Any multiple sequence alignment illustrates that, e.g., the feature hydrophobicity is more conserved than is the residue type. For the million protein pairs investigated, here, this was reflected in a shift of the scatter plot towards lower percentages. The original HSSP-curve was derived for sequence identity, not for sequence similarity (Sander & Schneider, 1991). Nevertheless, the curve was clearly more conservative in terms of excluding false positives for similarity than for identity. The major problem was the saturation at levels of 25% sequence identity. Indeed, for longer alignments false positives fall below about 15% pairwise sequence similarity. The principle functional dependence between similarity and length appeared similar to the one between identity and length. This prompted a similar definition for the separation between true and false positives based on similarity:
(3)
where L gave the number of residues aligned between two
proteins; pS defined cut-off for
the percentage of residue similarity over the L aligned
residues; and n described the distance in percentage points
from the curve.
The cumulative number of protein pairs with similar structure detected at the level of significant sequence identity is:
To obtain levels of 99% true positives you have to add N percentage points to the values given below, with N being:
| Number of residues aligned | Significant sequence identity (eq. 1) | Significant sequence identity (eq. 2) | Significant sequence similarity (eq. 3) |
| 1 | 100.0 | 100.0 | 100.0 |
| 2 | 100.0 | 100.0 | 100.0 |
| 3 | 100.0 | 100.0 | 100.0 |
| 4 | 100.0 | 100.0 | 100.0 |
| 5 | 100.0 | 100.0 | 100.0 |
| 6 | 100.0 | 100.0 | 100.0 |
| 7 | 97.2 | 100.0 | 100.0 |
| 8 | 90.2 | 100.0 | 100.0 |
| 9 | 84.4 | 100.0 | 96.7 |
| 10 | 79.5 | 100.0 | 90.1 |
| 11 | 75.4 | 100.0 | 84.6 |
| 12 | 71.8 | 98.8 | 79.9 |
| 13 | 68.6 | 94.0 | 75.7 |
| 14 | 65.8 | 89.7 | 72.1 |
| 15 | 63.3 | 85.9 | 68.9 |
| 16 | 61.1 | 82.6 | 66.0 |
| 17 | 59.0 | 79.5 | 63.4 |
| 18 | 57.2 | 76.7 | 61.1 |
| 19 | 55.5 | 74.2 | 59.0 |
| 20 | 53.9 | 71.9 | 57.0 |
| 21 | 52.4 | 69.8 | 55.2 |
| 22 | 51.1 | 67.8 | 53.5 |
| 23 | 49.8 | 66.0 | 52.0 |
| 24 | 48.6 | 64.3 | 50.6 |
| 25 | 47.5 | 62.7 | 49.3 |
| 26 | 46.5 | 61.3 | 48.0 |
| 27 | 45.5 | 59.9 | 46.8 |
| 28 | 44.6 | 58.6 | 45.8 |
| 29 | 43.7 | 57.4 | 44.7 |
| 30 | 42.9 | 56.2 | 43.7 |
| 31 | 42.1 | 55.1 | 42.8 |
| 32 | 41.4 | 54.1 | 42.0 |
| 33 | 40.7 | 53.1 | 41.1 |
| 34 | 40.0 | 52.2 | 40.3 |
| 35 | 39.3 | 51.3 | 39.6 |
| 36 | 38.7 | 50.4 | 38.9 |
| 37 | 38.1 | 49.6 | 38.2 |
| 38 | 37.6 | 48.9 | 37.6 |
| 39 | 37.0 | 48.1 | 36.9 |
| 40 | 36.5 | 47.4 | 36.3 |
| 41 | 36.0 | 46.8 | 35.8 |
| 42 | 35.5 | 46.1 | 35.2 |
| 43 | 35.0 | 45.5 | 34.7 |
| 44 | 34.6 | 44.9 | 34.2 |
| 45 | 34.2 | 44.3 | 33.7 |
| 46 | 33.7 | 43.8 | 33.3 |
| 47 | 33.3 | 43.2 | 32.8 |
| 48 | 32.9 | 42.7 | 32.4 |
| 49 | 32.6 | 42.2 | 32.0 |
| 50 | 32.2 | 41.7 | 31.5 |
| 51 | 31.8 | 41.3 | 31.2 |
| 52 | 31.5 | 40.8 | 30.8 |
| 53 | 31.2 | 40.4 | 30.4 |
| 54 | 30.8 | 40.0 | 30.1 |
| 55 | 30.5 | 39.6 | 29.7 |
| 56 | 30.2 | 39.2 | 29.4 |
| 57 | 29.9 | 38.8 | 29.1 |
| 58 | 29.6 | 38.4 | 28.8 |
| 59 | 29.3 | 38.1 | 28.4 |
| 60 | 29.1 | 37.7 | 28.2 |
| 61 | 28.8 | 37.4 | 27.9 |
| 62 | 28.5 | 37.0 | 27.6 |
| 63 | 28.3 | 36.7 | 27.3 |
| 64 | 28.0 | 36.4 | 27.0 |
| 65 | 27.8 | 36.1 | 26.8 |
| 66 | 27.5 | 35.8 | 26.5 |
| 67 | 27.3 | 35.5 | 26.3 |
| 68 | 27.1 | 35.2 | 26.1 |
| 69 | 26.9 | 35.0 | 25.8 |
| 70 | 26.6 | 34.7 | 25.6 |
| 71 | 26.4 | 34.4 | 25.4 |
| 72 | 26.2 | 34.2 | 25.2 |
| 73 | 26.0 | 33.9 | 25.0 |
| 74 | 25.8 | 33.7 | 24.8 |
| 75 | 25.6 | 33.5 | 24.6 |
| 76 | 25.4 | 33.2 | 24.4 |
| 77 | 25.3 | 33.0 | 24.2 |
| 78 | 25.1 | 32.8 | 24.0 |
| 79 | 24.9 | 32.6 | 23.8 |
| 80 | 24.7 | 32.4 | 23.6 |
| 81 | 25.0 | 32.2 | 23.4 |
| 82 | 25.0 | 32.0 | 23.3 |
| 83 | 25.0 | 31.8 | 23.1 |
| 84 | 25.0 | 31.6 | 22.9 |
| 85 | 25.0 | 31.4 | 22.8 |
| 86 | 25.0 | 31.2 | 22.6 |
| 87 | 25.0 | 31.0 | 22.5 |
| 88 | 25.0 | 30.8 | 22.3 |
| 89 | 25.0 | 30.7 | 22.2 |
| 90 | 25.0 | 30.5 | 22.0 |
| 91 | 25.0 | 30.3 | 21.9 |
| 92 | 25.0 | 30.2 | 21.7 |
| 93 | 25.0 | 30.0 | 21.6 |
| 94 | 25.0 | 29.9 | 21.5 |
| 95 | 25.0 | 29.7 | 21.3 |
| 96 | 25.0 | 29.6 | 21.2 |
| 97 | 25.0 | 29.4 | 21.1 |
| 98 | 25.0 | 29.3 | 20.9 |
| 99 | 25.0 | 29.1 | 20.8 |
| 100 | 25.0 | 29.0 | 20.7 |
| 101 | 25.0 | 28.8 | 20.6 |
| 102 | 25.0 | 28.7 | 20.5 |
| 103 | 25.0 | 28.6 | 20.3 |
| 104 | 25.0 | 28.5 | 20.2 |
| 105 | 25.0 | 28.3 | 20.1 |
| 106 | 25.0 | 28.2 | 20.0 |
| 107 | 25.0 | 28.1 | 19.9 |
| 108 | 25.0 | 28.0 | 19.8 |
| 109 | 25.0 | 27.8 | 19.7 |
| 110 | 25.0 | 27.7 | 19.6 |
| 111 | 25.0 | 27.6 | 19.5 |
| 112 | 25.0 | 27.5 | 19.4 |
| 113 | 25.0 | 27.4 | 19.3 |
| 114 | 25.0 | 27.3 | 19.2 |
| 115 | 25.0 | 27.2 | 19.1 |
| 116 | 25.0 | 27.1 | 19.0 |
| 117 | 25.0 | 27.0 | 18.9 |
| 118 | 25.0 | 26.9 | 18.8 |
| 119 | 25.0 | 26.8 | 18.7 |
| 120 | 25.0 | 26.7 | 18.7 |
| 121 | 25.0 | 26.6 | 18.6 |
| 122 | 25.0 | 26.5 | 18.5 |
| 123 | 25.0 | 26.4 | 18.4 |
| 124 | 25.0 | 26.3 | 18.3 |
| 125 | 25.0 | 26.2 | 18.2 |
| 126 | 25.0 | 26.1 | 18.2 |
| 127 | 25.0 | 26.0 | 18.1 |
| 128 | 25.0 | 25.9 | 18.0 |
| 129 | 25.0 | 25.8 | 17.9 |
| 130 | 25.0 | 25.8 | 17.8 |
| 131 | 25.0 | 25.7 | 17.8 |
| 132 | 25.0 | 25.6 | 17.7 |
| 133 | 25.0 | 25.5 | 17.6 |
| 134 | 25.0 | 25.4 | 17.5 |
| 135 | 25.0 | 25.3 | 17.5 |
| 136 | 25.0 | 25.3 | 17.4 |
| 137 | 25.0 | 25.2 | 17.3 |
| 138 | 25.0 | 25.1 | 17.3 |
| 139 | 25.0 | 25.0 | 17.2 |
| 140 | 25.0 | 25.0 | 17.1 |
| 141 | 25.0 | 24.9 | 17.1 |
| 142 | 25.0 | 24.8 | 17.0 |
| 143 | 25.0 | 24.8 | 16.9 |
| 144 | 25.0 | 24.7 | 16.9 |
| 145 | 25.0 | 24.6 | 16.8 |
| 146 | 25.0 | 24.6 | 16.8 |
| 147 | 25.0 | 24.5 | 16.7 |
| 148 | 25.0 | 24.4 | 16.6 |
| 149 | 25.0 | 24.4 | 16.6 |
| 150 | 25.0 | 24.3 | 16.5 |
| 151 | 25.0 | 24.2 | 16.5 |
| 152 | 25.0 | 24.2 | 16.4 |
| 153 | 25.0 | 24.1 | 16.3 |
| 154 | 25.0 | 24.1 | 16.3 |
| 155 | 25.0 | 24.0 | 16.2 |
| 156 | 25.0 | 23.9 | 16.2 |
| 157 | 25.0 | 23.9 | 16.1 |
| 158 | 25.0 | 23.8 | 16.1 |
| 159 | 25.0 | 23.8 | 16.0 |
| 160 | 25.0 | 23.7 | 16.0 |
| 161 | 25.0 | 23.7 | 15.9 |
| 162 | 25.0 | 23.6 | 15.9 |
| 163 | 25.0 | 23.5 | 15.8 |
| 164 | 25.0 | 23.5 | 15.8 |
| 165 | 25.0 | 23.4 | 15.7 |
| 166 | 25.0 | 23.4 | 15.7 |
| 167 | 25.0 | 23.3 | 15.6 |
| 168 | 25.0 | 23.3 | 15.6 |
| 169 | 25.0 | 23.2 | 15.5 |
| 170 | 25.0 | 23.2 | 15.5 |
| 171 | 25.0 | 23.1 | 15.4 |
| 172 | 25.0 | 23.1 | 15.4 |
| 173 | 25.0 | 23.0 | 15.3 |
| 174 | 25.0 | 23.0 | 15.3 |
| 175 | 25.0 | 23.0 | 15.3 |
| 176 | 25.0 | 22.9 | 15.2 |
| 177 | 25.0 | 22.9 | 15.2 |
| 178 | 25.0 | 22.8 | 15.1 |
| 179 | 25.0 | 22.8 | 15.1 |
| 180 | 25.0 | 22.7 | 15.0 |
| 181 | 25.0 | 22.7 | 15.0 |
| 182 | 25.0 | 22.7 | 15.0 |
| 183 | 25.0 | 22.6 | 14.9 |
| 184 | 25.0 | 22.6 | 14.9 |
| 185 | 25.0 | 22.5 | 14.8 |
| 186 | 25.0 | 22.5 | 14.8 |
| 187 | 25.0 | 22.5 | 14.8 |
| 188 | 25.0 | 22.4 | 14.7 |
| 189 | 25.0 | 22.4 | 14.7 |
| 190 | 25.0 | 22.3 | 14.6 |
| 191 | 25.0 | 22.3 | 14.6 |
| 192 | 25.0 | 22.3 | 14.6 |
| 193 | 25.0 | 22.2 | 14.5 |
| 194 | 25.0 | 22.2 | 14.5 |
| 195 | 25.0 | 22.2 | 14.5 |
| 196 | 25.0 | 22.1 | 14.4 |
| 197 | 25.0 | 22.1 | 14.4 |
| 198 | 25.0 | 22.0 | 14.4 |
| 199 | 25.0 | 22.0 | 14.3 |
| 200 | 25.0 | 22.0 | 14.3 |
| 201 | 25.0 | 21.9 | 14.3 |
| 202 | 25.0 | 21.9 | 14.2 |
| 203 | 25.0 | 21.9 | 14.2 |
| 204 | 25.0 | 21.9 | 14.2 |
| 205 | 25.0 | 21.8 | 14.1 |
| 206 | 25.0 | 21.8 | 14.1 |
| 207 | 25.0 | 21.8 | 14.1 |
| 208 | 25.0 | 21.7 | 14.0 |
| 209 | 25.0 | 21.7 | 14.0 |
| 210 | 25.0 | 21.7 | 14.0 |
| 211 | 25.0 | 21.6 | 13.9 |
| 212 | 25.0 | 21.6 | 13.9 |
| 213 | 25.0 | 21.6 | 13.9 |
| 214 | 25.0 | 21.5 | 13.8 |
| 215 | 25.0 | 21.5 | 13.8 |
| 216 | 25.0 | 21.5 | 13.8 |
| 217 | 25.0 | 21.5 | 13.7 |
| 218 | 25.0 | 21.4 | 13.7 |
| 219 | 25.0 | 21.4 | 13.7 |
| 220 | 25.0 | 21.4 | 13.7 |
| 221 | 25.0 | 21.4 | 13.6 |
| 222 | 25.0 | 21.3 | 13.6 |
| 223 | 25.0 | 21.3 | 13.6 |
| 224 | 25.0 | 21.3 | 13.6 |
| 225 | 25.0 | 21.3 | 13.5 |
| 226 | 25.0 | 21.2 | 13.5 |
| 227 | 25.0 | 21.2 | 13.5 |
| 228 | 25.0 | 21.2 | 13.4 |
| 229 | 25.0 | 21.2 | 13.4 |
| 230 | 25.0 | 21.1 | 13.4 |
| 231 | 25.0 | 21.1 | 13.4 |
| 232 | 25.0 | 21.1 | 13.3 |
| 233 | 25.0 | 21.1 | 13.3 |
| 234 | 25.0 | 21.0 | 13.3 |
| 235 | 25.0 | 21.0 | 13.3 |
| 236 | 25.0 | 21.0 | 13.2 |
| 237 | 25.0 | 21.0 | 13.2 |
| 238 | 25.0 | 21.0 | 13.2 |
| 239 | 25.0 | 20.9 | 13.2 |
| 240 | 25.0 | 20.9 | 13.1 |
| 241 | 25.0 | 20.9 | 13.1 |
| 242 | 25.0 | 20.9 | 13.1 |
| 243 | 25.0 | 20.9 | 13.1 |
| 244 | 25.0 | 20.8 | 13.0 |
| 245 | 25.0 | 20.8 | 13.0 |
| 246 | 25.0 | 20.8 | 13.0 |
| 247 | 25.0 | 20.8 | 13.0 |
| 248 | 25.0 | 20.8 | 13.0 |
| 249 | 25.0 | 20.7 | 12.9 |
| 250 | 25.0 | 20.7 | 12.9 |
| 251 | 25.0 | 20.7 | 12.9 |
| 252 | 25.0 | 20.7 | 12.9 |
| 253 | 25.0 | 20.7 | 12.8 |
| 254 | 25.0 | 20.6 | 12.8 |
| 255 | 25.0 | 20.6 | 12.8 |
| 256 | 25.0 | 20.6 | 12.8 |
| 257 | 25.0 | 20.6 | 12.8 |
| 258 | 25.0 | 20.6 | 12.7 |
| 259 | 25.0 | 20.6 | 12.7 |
| 260 | 25.0 | 20.5 | 12.7 |
| 261 | 25.0 | 20.5 | 12.7 |
| 262 | 25.0 | 20.5 | 12.7 |
| 263 | 25.0 | 20.5 | 12.6 |
| 264 | 25.0 | 20.5 | 12.6 |
| 265 | 25.0 | 20.5 | 12.6 |
| 266 | 25.0 | 20.4 | 12.6 |
| 267 | 25.0 | 20.4 | 12.6 |
| 268 | 25.0 | 20.4 | 12.5 |
| 269 | 25.0 | 20.4 | 12.5 |
| 270 | 25.0 | 20.4 | 12.5 |
| 271 | 25.0 | 20.4 | 12.5 |
| 272 | 25.0 | 20.4 | 12.5 |
| 273 | 25.0 | 20.3 | 12.4 |
| 274 | 25.0 | 20.3 | 12.4 |
| 275 | 25.0 | 20.3 | 12.4 |
| 276 | 25.0 | 20.3 | 12.4 |
| 277 | 25.0 | 20.3 | 12.4 |
| 278 | 25.0 | 20.3 | 12.4 |
| 279 | 25.0 | 20.3 | 12.3 |
| 280 | 25.0 | 20.2 | 12.3 |
| 281 | 25.0 | 20.2 | 12.3 |
| 282 | 25.0 | 20.2 | 12.3 |
| 283 | 25.0 | 20.2 | 12.3 |
| 284 | 25.0 | 20.2 | 12.3 |
| 285 | 25.0 | 20.2 | 12.2 |
| 286 | 25.0 | 20.2 | 12.2 |
| 287 | 25.0 | 20.2 | 12.2 |
| 288 | 25.0 | 20.1 | 12.2 |
| 289 | 25.0 | 20.1 | 12.2 |
| 290 | 25.0 | 20.1 | 12.2 |
| 291 | 25.0 | 20.1 | 12.1 |
| 292 | 25.0 | 20.1 | 12.1 |
| 293 | 25.0 | 20.1 | 12.1 |
| 294 | 25.0 | 20.1 | 12.1 |
| 295 | 25.0 | 20.1 | 12.1 |
| 296 | 25.0 | 20.1 | 12.1 |
| 297 | 25.0 | 20.0 | 12.0 |
| 298 | 25.0 | 20.0 | 12.0 |
| 299 | 25.0 | 20.0 | 12.0 |
| 300 | 25.0 | 20.0 | 12.0 |
| 301 | 25.0 | 20.0 | 12.0 |
| 302 | 25.0 | 20.0 | 12.0 |
| 303 | 25.0 | 20.0 | 12.0 |
| 304 | 25.0 | 20.0 | 11.9 |
| 305 | 25.0 | 20.0 | 11.9 |
| 306 | 25.0 | 20.0 | 11.9 |
| 307 | 25.0 | 19.9 | 11.9 |
| 308 | 25.0 | 19.9 | 11.9 |
| 309 | 25.0 | 19.9 | 11.9 |
| 310 | 25.0 | 19.9 | 11.9 |
| 311 | 25.0 | 19.9 | 11.8 |
| 312 | 25.0 | 19.9 | 11.8 |
| 313 | 25.0 | 19.9 | 11.8 |
| 314 | 25.0 | 19.9 | 11.8 |
| 315 | 25.0 | 19.9 | 11.8 |
| 316 | 25.0 | 19.9 | 11.8 |
| 317 | 25.0 | 19.9 | 11.8 |
| 318 | 25.0 | 19.9 | 11.7 |
| 319 | 25.0 | 19.8 | 11.7 |
| 320 | 25.0 | 19.8 | 11.7 |
| 321 | 25.0 | 19.8 | 11.7 |
| 322 | 25.0 | 19.8 | 11.7 |
| 323 | 25.0 | 19.8 | 11.7 |
| 324 | 25.0 | 19.8 | 11.7 |
| 325 | 25.0 | 19.8 | 11.7 |
| 326 | 25.0 | 19.8 | 11.6 |
| 327 | 25.0 | 19.8 | 11.6 |
| 328 | 25.0 | 19.8 | 11.6 |
| 329 | 25.0 | 19.8 | 11.6 |
| 330 | 25.0 | 19.8 | 11.6 |
| 331 | 25.0 | 19.8 | 11.6 |
| 332 | 25.0 | 19.8 | 11.6 |
| 333 | 25.0 | 19.7 | 11.6 |
| 334 | 25.0 | 19.7 | 11.5 |
| 335 | 25.0 | 19.7 | 11.5 |
| 336 | 25.0 | 19.7 | 11.5 |
| 337 | 25.0 | 19.7 | 11.5 |
| 338 | 25.0 | 19.7 | 11.5 |
| 339 | 25.0 | 19.7 | 11.5 |
| 340 | 25.0 | 19.7 | 11.5 |
| 341 | 25.0 | 19.7 | 11.5 |
| 342 | 25.0 | 19.7 | 11.5 |
| 343 | 25.0 | 19.7 | 11.4 |
| 344 | 25.0 | 19.7 | 11.4 |
| 345 | 25.0 | 19.7 | 11.4 |
| 346 | 25.0 | 19.7 | 11.4 |
| 347 | 25.0 | 19.7 | 11.4 |
| 348 | 25.0 | 19.7 | 11.4 |
| 349 | 25.0 | 19.7 | 11.4 |
| 350 | 25.0 | 19.7 | 11.4 |
| 351 | 25.0 | 19.6 | 11.4 |
| 352 | 25.0 | 19.6 | 11.3 |
| 353 | 25.0 | 19.6 | 11.3 |
| 354 | 25.0 | 19.6 | 11.3 |
| 355 | 25.0 | 19.6 | 11.3 |
| 356 | 25.0 | 19.6 | 11.3 |
| 357 | 25.0 | 19.6 | 11.3 |
| 358 | 25.0 | 19.6 | 11.3 |
| 359 | 25.0 | 19.6 | 11.3 |
| 360 | 25.0 | 19.6 | 11.3 |
| 361 | 25.0 | 19.6 | 11.3 |
| 362 | 25.0 | 19.6 | 11.2 |
| 363 | 25.0 | 19.6 | 11.2 |
| 364 | 25.0 | 19.6 | 11.2 |
| 365 | 25.0 | 19.6 | 11.2 |
| 366 | 25.0 | 19.6 | 11.2 |
| 367 | 25.0 | 19.6 | 11.2 |
| 368 | 25.0 | 19.6 | 11.2 |
| 369 | 25.0 | 19.6 | 11.2 |
| 370 | 25.0 | 19.6 | 11.2 |
| 371 | 25.0 | 19.6 | 11.2 |
| 372 | 25.0 | 19.6 | 11.1 |
| 373 | 25.0 | 19.6 | 11.1 |
| 374 | 25.0 | 19.6 | 11.1 |
| 375 | 25.0 | 19.6 | 11.1 |
| 376 | 25.0 | 19.6 | 11.1 |
| 377 | 25.0 | 19.6 | 11.1 |
| 378 | 25.0 | 19.6 | 11.1 |
| 379 | 25.0 | 19.6 | 11.1 |
| 380 | 25.0 | 19.6 | 11.1 |
| 381 | 25.0 | 19.5 | 11.1 |
| 382 | 25.0 | 19.5 | 11.1 |
| 383 | 25.0 | 19.5 | 11.0 |
| 384 | 25.0 | 19.5 | 11.0 |
| 385 | 25.0 | 19.5 | 11.0 |
| 386 | 25.0 | 19.5 | 11.0 |
| 387 | 25.0 | 19.5 | 11.0 |
| 388 | 25.0 | 19.5 | 11.0 |
| 389 | 25.0 | 19.5 | 11.0 |
| 390 | 25.0 | 19.5 | 11.0 |
| 391 | 25.0 | 19.5 | 11.0 |
| 392 | 25.0 | 19.5 | 11.0 |
| 393 | 25.0 | 19.5 | 11.0 |
| 394 | 25.0 | 19.5 | 11.0 |
| 395 | 25.0 | 19.5 | 11.0 |
| 396 | 25.0 | 19.5 | 10.9 |
| 397 | 25.0 | 19.5 | 10.9 |
| 398 | 25.0 | 19.5 | 10.9 |
| 399 | 25.0 | 19.5 | 10.9 |
| 400 | 25.0 | 19.5 | 10.9 |
| 401 | 25.0 | 19.5 | 10.9 |
| 402 | 25.0 | 19.5 | 10.9 |
| 403 | 25.0 | 19.5 | 10.9 |
| 404 | 25.0 | 19.5 | 10.9 |
| 405 | 25.0 | 19.5 | 10.9 |
| 406 | 25.0 | 19.5 | 10.9 |
| 407 | 25.0 | 19.5 | 10.9 |
| 408 | 25.0 | 19.5 | 10.9 |
| 409 | 25.0 | 19.5 | 10.8 |
| 410 | 25.0 | 19.5 | 10.8 |
| 411 | 25.0 | 19.5 | 10.8 |
| 412 | 25.0 | 19.5 | 10.8 |
| 413 | 25.0 | 19.5 | 10.8 |
| 414 | 25.0 | 19.5 | 10.8 |
| 415 | 25.0 | 19.5 | 10.8 |
| 416 | 25.0 | 19.5 | 10.8 |
| 417 | 25.0 | 19.5 | 10.8 |
| 418 | 25.0 | 19.5 | 10.8 |
| 419 | 25.0 | 19.5 | 10.8 |
| 420 | 25.0 | 19.5 | 10.8 |
| 421 | 25.0 | 19.5 | 10.8 |
| 422 | 25.0 | 19.5 | 10.8 |
| 423 | 25.0 | 19.5 | 10.7 |
| 424 | 25.0 | 19.5 | 10.7 |
| 425 | 25.0 | 19.5 | 10.7 |
| 426 | 25.0 | 19.5 | 10.7 |
| 427 | 25.0 | 19.5 | 10.7 |
| 428 | 25.0 | 19.5 | 10.7 |
| 429 | 25.0 | 19.5 | 10.7 |
| 430 | 25.0 | 19.5 | 10.7 |
| 431 | 25.0 | 19.5 | 10.7 |
| 432 | 25.0 | 19.5 | 10.7 |
| 433 | 25.0 | 19.5 | 10.7 |
| 434 | 25.0 | 19.5 | 10.7 |
| 435 | 25.0 | 19.5 | 10.7 |
| 436 | 25.0 | 19.5 | 10.7 |
| 437 | 25.0 | 19.5 | 10.7 |
| 438 | 25.0 | 19.5 | 10.7 |
| 439 | 25.0 | 19.5 | 10.6 |
| 440 | 25.0 | 19.5 | 10.6 |
| 441 | 25.0 | 19.5 | 10.6 |
| 442 | 25.0 | 19.5 | 10.6 |
| 443 | 25.0 | 19.5 | 10.6 |
| 444 | 25.0 | 19.5 | 10.6 |
| 445 | 25.0 | 19.5 | 10.6 |
| 446 | 25.0 | 19.5 | 10.6 |
| 447 | 25.0 | 19.5 | 10.6 |
| 448 | 25.0 | 19.5 | 10.6 |
| 449 | 25.0 | 19.5 | 10.6 |
| 450 | 25.0 | 19.5 | 10.6 |
| 451 | 25.0 | 19.5 | 10.6 |
| 452 | 25.0 | 19.5 | 10.6 |
| 453 | 25.0 | 19.5 | 10.6 |
| 454 | 25.0 | 19.5 | 10.6 |
| 455 | 25.0 | 19.5 | 10.6 |
| 456 | 25.0 | 19.5 | 10.6 |
| 457 | 25.0 | 19.5 | 10.5 |
| 458 | 25.0 | 19.5 | 10.5 |
| 459 | 25.0 | 19.5 | 10.5 |
| 460 | 25.0 | 19.5 | 10.5 |
| 461 | 25.0 | 19.5 | 10.5 |
| 462 | 25.0 | 19.5 | 10.5 |
| 463 | 25.0 | 19.5 | 10.5 |
| 464 | 25.0 | 19.5 | 10.5 |
| 465 | 25.0 | 19.5 | 10.5 |
| 466 | 25.0 | 19.5 | 10.5 |
| 467 | 25.0 | 19.5 | 10.5 |
| 468 | 25.0 | 19.5 | 10.5 |
| 469 | 25.0 | 19.5 | 10.5 |
| 470 | 25.0 | 19.5 | 10.5 |
| 471 | 25.0 | 19.5 | 10.5 |
| 472 | 25.0 | 19.5 | 10.5 |
| 473 | 25.0 | 19.5 | 10.5 |
| 474 | 25.0 | 19.5 | 10.5 |
| 475 | 25.0 | 19.5 | 10.5 |
| 476 | 25.0 | 19.5 | 10.5 |
| 477 | 25.0 | 19.5 | 10.4 |
| 478 | 25.0 | 19.5 | 10.4 |
| 479 | 25.0 | 19.5 | 10.4 |
| 480 | 25.0 | 19.5 | 10.4 |
| 481 | 25.0 | 19.5 | 10.4 |
| 482 | 25.0 | 19.5 | 10.4 |
| 483 | 25.0 | 19.5 | 10.4 |
| 484 | 25.0 | 19.5 | 10.4 |
| 485 | 25.0 | 19.5 | 10.4 |
| 486 | 25.0 | 19.5 | 10.4 |
| 487 | 25.0 | 19.5 | 10.4 |
| 488 | 25.0 | 19.5 | 10.4 |
| 489 | 25.0 | 19.5 | 10.4 |
| 490 | 25.0 | 19.5 | 10.4 |
| 491 | 25.0 | 19.5 | 10.4 |
| 492 | 25.0 | 19.5 | 10.4 |
| 493 | 25.0 | 19.5 | 10.4 |
| 494 | 25.0 | 19.5 | 10.4 |
| 495 | 25.0 | 19.5 | 10.4 |
| 496 | 25.0 | 19.5 | 10.4 |
| 497 | 25.0 | 19.5 | 10.4 |
| 498 | 25.0 | 19.5 | 10.4 |
| 499 | 25.0 | 19.5 | 10.4 |
| 500 | 25.0 | 19.5 | 10.3 |
| 501 | 25.0 | 19.5 | 10.3 |
| 502 | 25.0 | 19.5 | 10.3 |
| 503 | 25.0 | 19.5 | 10.3 |
| 504 | 25.0 | 19.5 | 10.3 |
| 505 | 25.0 | 19.5 | 10.3 |
| 506 | 25.0 | 19.5 | 10.3 |
| 507 | 25.0 | 19.5 | 10.3 |
| 508 | 25.0 | 19.5 | 10.3 |
| 509 | 25.0 | 19.5 | 10.3 |
| 510 | 25.0 | 19.5 | 10.3 |
| 511 | 25.0 | 19.5 | 10.3 |
| 512 | 25.0 | 19.5 | 10.3 |
| 513 | 25.0 | 19.5 | 10.3 |
| 514 | 25.0 | 19.5 | 10.3 |
| 515 | 25.0 | 19.5 | 10.3 |
| 516 | 25.0 | 19.5 | 10.3 |
| 517 | 25.0 | 19.5 | 10.3 |
| 518 | 25.0 | 19.5 | 10.3 |
| 519 | 25.0 | 19.5 | 10.3 |
| 520 | 25.0 | 19.5 | 10.3 |
| 521 | 25.0 | 19.5 | 10.3 |
| 522 | 25.0 | 19.5 | 10.3 |
| 523 | 25.0 | 19.5 | 10.3 |
| 524 | 25.0 | 19.5 | 10.3 |
| 525 | 25.0 | 19.5 | 10.3 |
| 526 | 25.0 | 19.5 | 10.3 |
| 527 | 25.0 | 19.5 | 10.3 |
| 528 | 25.0 | 19.5 | 10.2 |
| 529 | 25.0 | 19.5 | 10.2 |
| 530 | 25.0 | 19.5 | 10.2 |
| 531 | 25.0 | 19.5 | 10.2 |
| 532 | 25.0 | 19.5 | 10.2 |
| 533 | 25.0 | 19.5 | 10.2 |
| 534 | 25.0 | 19.5 | 10.2 |
| 535 | 25.0 | 19.5 | 10.2 |
| 536 | 25.0 | 19.5 | 10.2 |
| 537 | 25.0 | 19.5 | 10.2 |
| 538 | 25.0 | 19.5 | 10.2 |
| 539 | 25.0 | 19.5 | 10.2 |
| 540 | 25.0 | 19.5 | 10.2 |
| 541 | 25.0 | 19.5 | 10.2 |
| 542 | 25.0 | 19.5 | 10.2 |
| 543 | 25.0 | 19.5 | 10.2 |
| 544 | 25.0 | 19.5 | 10.2 |
| 545 | 25.0 | 19.5 | 10.2 |
| 546 | 25.0 | 19.5 | 10.2 |
| 547 | 25.0 | 19.5 | 10.2 |
| 548 | 25.0 | 19.5 | 10.2 |
| 549 | 25.0 | 19.5 | 10.2 |
| 550 | 25.0 | 19.5 | 10.2 |
| 551 | 25.0 | 19.5 | 10.2 |
| 552 | 25.0 | 19.5 | 10.2 |
| 553 | 25.0 | 19.5 | 10.2 |
| 554 | 25.0 | 19.5 | 10.2 |
| 555 | 25.0 | 19.5 | 10.2 |
| 556 | 25.0 | 19.5 | 10.2 |
| 557 | 25.0 | 19.5 | 10.2 |
| 558 | 25.0 | 19.5 | 10.2 |
| 559 | 25.0 | 19.5 | 10.2 |
| 560 | 25.0 | 19.5 | 10.2 |
| 561 | 25.0 | 19.5 | 10.2 |
| 562 | 25.0 | 19.5 | 10.2 |
| 563 | 25.0 | 19.5 | 10.2 |
| 564 | 25.0 | 19.5 | 10.1 |
| 565 | 25.0 | 19.5 | 10.1 |
| 566 | 25.0 | 19.5 | 10.1 |
| 567 | 25.0 | 19.5 | 10.1 |
| 568 | 25.0 | 19.5 | 10.1 |
| 569 | 25.0 | 19.5 | 10.1 |
| 570 | 25.0 | 19.5 | 10.1 |
| 571 | 25.0 | 19.5 | 10.1 |
| 572 | 25.0 | 19.5 | 10.1 |
| 573 | 25.0 | 19.5 | 10.1 |
| 574 | 25.0 | 19.5 | 10.1 |
| 575 | 25.0 | 19.5 | 10.1 |
| 576 | 25.0 | 19.5 | 10.1 |
| 577 | 25.0 | 19.5 | 10.1 |
| 578 | 25.0 | 19.5 | 10.1 |
| 579 | 25.0 | 19.5 | 10.1 |
| 580 | 25.0 | 19.5 | 10.1 |
| 581 | 25.0 | 19.5 | 10.1 |
| 582 | 25.0 | 19.5 | 10.1 |
| 583 | 25.0 | 19.5 | 10.1 |
| 584 | 25.0 | 19.5 | 10.1 |
| 585 | 25.0 | 19.5 | 10.1 |
| 586 | 25.0 | 19.5 | 10.1 |
| 587 | 25.0 | 19.5 | 10.1 |
| 588 | 25.0 | 19.5 | 10.1 |
| 589 | 25.0 | 19.5 | 10.1 |
| 590 | 25.0 | 19.5 | 10.1 |
| 591 | 25.0 | 19.5 | 10.1 |
| 592 | 25.0 | 19.5 | 10.1 |
| 593 | 25.0 | 19.5 | 10.1 |
| 594 | 25.0 | 19.5 | 10.1 |
| 595 | 25.0 | 19.5 | 10.1 |
| 596 | 25.0 | 19.5 | 10.1 |
| 597 | 25.0 | 19.5 | 10.1 |
| 598 | 25.0 | 19.5 | 10.1 |
| 599 | 25.0 | 19.5 | 10.1 |
| 600 | 25.0 | 19.5 | 10.1 |
| 601 | 25.0 | 19.5 | 10.1 |
| 602 | 25.0 | 19.5 | 10.1 |
| 603 | 25.0 | 19.5 | 10.1 |
| 604 | 25.0 | 19.5 | 10.1 |
| 605 | 25.0 | 19.5 | 10.1 |
| 606 | 25.0 | 19.5 | 10.1 |
| 607 | 25.0 | 19.5 | 10.1 |
| 608 | 25.0 | 19.5 | 10.1 |
| 609 | 25.0 | 19.5 | 10.1 |
| 610 | 25.0 | 19.5 | 10.1 |
| 611 | 25.0 | 19.5 | 10.1 |
| 612 | 25.0 | 19.5 | 10.1 |
| 613 | 25.0 | 19.5 | 10.0 |
| 614 | 25.0 | 19.5 | 10.0 |
| 615 | 25.0 | 19.5 | 10.0 |
| 616 | 25.0 | 19.5 | 10.0 |
| 617 | 25.0 | 19.5 | 10.0 |
| 618 | 25.0 | 19.5 | 10.0 |
| 619 | 25.0 | 19.5 | 10.0 |
| 620 | 25.0 | 19.5 | 10.0 |
| 621 | 25.0 | 19.5 | 10.0 |
| 622 | 25.0 | 19.5 | 10.0 |
| 623 | 25.0 | 19.5 | 10.0 |
| 624 | 25.0 | 19.5 | 10.0 |
| 625 | 25.0 | 19.5 | 10.0 |
| 626 | 25.0 | 19.5 | 10.0 |
| 627 | 25.0 | 19.5 | 10.0 |
| 628 | 25.0 | 19.5 | 10.0 |
| 629 | 25.0 | 19.5 | 10.0 |
| 630 | 25.0 | 19.5 | 10.0 |
| 631 | 25.0 | 19.5 | 10.0 |
| 632 | 25.0 | 19.5 | 10.0 |
| 633 | 25.0 | 19.5 | 10.0 |
| 634 | 25.0 | 19.5 | 10.0 |
| 635 | 25.0 | 19.5 | 10.0 |
| 636 | 25.0 | 19.5 | 10.0 |
| 637 | 25.0 | 19.5 | 10.0 |
| 638 | 25.0 | 19.5 | 10.0 |
| 639 | 25.0 | 19.5 | 10.0 |
| 640 | 25.0 | 19.5 | 10.0 |
| 641 | 25.0 | 19.5 | 10.0 |
| 642 | 25.0 | 19.5 | 10.0 |
| 643 | 25.0 | 19.5 | 10.0 |
| 644 | 25.0 | 19.5 | 10.0 |
| 645 | 25.0 | 19.5 | 10.0 |
| 646 | 25.0 | 19.5 | 10.0 |
| 647 | 25.0 | 19.5 | 10.0 |
| 648 | 25.0 | 19.5 | 10.0 |
| 649 | 25.0 | 19.5 | 10.0 |
| 650 | 25.0 | 19.5 | 10.0 |
| 651 | 25.0 | 19.5 | 10.0 |
| 652 | 25.0 | 19.5 | 10.0 |
| 653 | 25.0 | 19.5 | 10.0 |
| 654 | 25.0 | 19.5 | 10.0 |
| 655 | 25.0 | 19.5 | 10.0 |
| 656 | 25.0 | 19.5 | 10.0 |
| 657 | 25.0 | 19.5 | 10.0 |
| 658 | 25.0 | 19.5 | 10.0 |
| 659 | 25.0 | 19.5 | 10.0 |
| 660 | 25.0 | 19.5 | 10.0 |
| 661 | 25.0 | 19.5 | 10.0 |
| 662 | 25.0 | 19.5 | 10.0 |
| 663 | 25.0 | 19.5 | 10.0 |
| 664 | 25.0 | 19.5 | 10.0 |
| 665 | 25.0 | 19.5 | 10.0 |
| 666 | 25.0 | 19.5 | 10.0 |
